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control gene fopflash  (Upstate Biotechnology Inc)

 
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    Upstate Biotechnology Inc control gene fopflash
    Catabolic regulation of LRP5 mediates via β-catenin-Tcf/Lef signaling. <t>(A)</t> <t>Chondrocytes</t> were transfected with 1 μg of empty vector (EV) or pSPORT6- Lrp5 plus the TOPflash or <t>FOPflash</t> reporter constructs. After 24 hours, transcriptional activation by β-catenin was determined by luciferase reporter gene assays ( n = 7 independent experiments). (B) Chondrocytes obtained from wild-type (WT) and Lrp5 -/- mice were treated with 1 ng/ml interleukin 1β (IL-1β), 50 ng/ml Wnt3a or 500 ng/ml Wnt7a, and transcriptional activation by β-catenin was evaluated by luciferase reporter gene assays ( n = 6). Values are expressed as means ± SEM (* P < 0.005). (C) β-catenin and LRP5 expression levels in cartilage after sham operation or destabilization of the medial meniscus (DMM) surgery were determined by immunofluorescence microscopy. 4′,6-diamidino-2-phenylindole (DAPI) staining was used for visualization of nuclei. Scale bar: 20 μm. (D) β-catenin, matrix metalloproteinase 3 (MMP3) and MMP13 expression levels in undamaged (Normal) and damaged (osteoarthritis; OA) human osteoarthritic cartilage were examined by immunostaining. (E) and (F) β-catenin, MMP3 and MMP13 expression levels in spontaneous (aging-induced) osteoarthritic cartilage and DMM-induced osteoarthritic cartilage from Lrp5 -/- mice and their WT littermates were examined by immunohistochemistry. Scale bar: 50 μm.
    Control Gene Fopflash, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control gene fopflash/product/Upstate Biotechnology Inc
    Average 90 stars, based on 1 article reviews
    control gene fopflash - by Bioz Stars, 2026-06
    90/100 stars

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    1) Product Images from "Low-density lipoprotein receptor–related protein 5 governs Wnt-mediated osteoarthritic cartilage destruction"

    Article Title: Low-density lipoprotein receptor–related protein 5 governs Wnt-mediated osteoarthritic cartilage destruction

    Journal: Arthritis Research & Therapy

    doi: 10.1186/ar4466

    Catabolic regulation of LRP5 mediates via β-catenin-Tcf/Lef signaling. (A) Chondrocytes were transfected with 1 μg of empty vector (EV) or pSPORT6- Lrp5 plus the TOPflash or FOPflash reporter constructs. After 24 hours, transcriptional activation by β-catenin was determined by luciferase reporter gene assays ( n = 7 independent experiments). (B) Chondrocytes obtained from wild-type (WT) and Lrp5 -/- mice were treated with 1 ng/ml interleukin 1β (IL-1β), 50 ng/ml Wnt3a or 500 ng/ml Wnt7a, and transcriptional activation by β-catenin was evaluated by luciferase reporter gene assays ( n = 6). Values are expressed as means ± SEM (* P < 0.005). (C) β-catenin and LRP5 expression levels in cartilage after sham operation or destabilization of the medial meniscus (DMM) surgery were determined by immunofluorescence microscopy. 4′,6-diamidino-2-phenylindole (DAPI) staining was used for visualization of nuclei. Scale bar: 20 μm. (D) β-catenin, matrix metalloproteinase 3 (MMP3) and MMP13 expression levels in undamaged (Normal) and damaged (osteoarthritis; OA) human osteoarthritic cartilage were examined by immunostaining. (E) and (F) β-catenin, MMP3 and MMP13 expression levels in spontaneous (aging-induced) osteoarthritic cartilage and DMM-induced osteoarthritic cartilage from Lrp5 -/- mice and their WT littermates were examined by immunohistochemistry. Scale bar: 50 μm.
    Figure Legend Snippet: Catabolic regulation of LRP5 mediates via β-catenin-Tcf/Lef signaling. (A) Chondrocytes were transfected with 1 μg of empty vector (EV) or pSPORT6- Lrp5 plus the TOPflash or FOPflash reporter constructs. After 24 hours, transcriptional activation by β-catenin was determined by luciferase reporter gene assays ( n = 7 independent experiments). (B) Chondrocytes obtained from wild-type (WT) and Lrp5 -/- mice were treated with 1 ng/ml interleukin 1β (IL-1β), 50 ng/ml Wnt3a or 500 ng/ml Wnt7a, and transcriptional activation by β-catenin was evaluated by luciferase reporter gene assays ( n = 6). Values are expressed as means ± SEM (* P < 0.005). (C) β-catenin and LRP5 expression levels in cartilage after sham operation or destabilization of the medial meniscus (DMM) surgery were determined by immunofluorescence microscopy. 4′,6-diamidino-2-phenylindole (DAPI) staining was used for visualization of nuclei. Scale bar: 20 μm. (D) β-catenin, matrix metalloproteinase 3 (MMP3) and MMP13 expression levels in undamaged (Normal) and damaged (osteoarthritis; OA) human osteoarthritic cartilage were examined by immunostaining. (E) and (F) β-catenin, MMP3 and MMP13 expression levels in spontaneous (aging-induced) osteoarthritic cartilage and DMM-induced osteoarthritic cartilage from Lrp5 -/- mice and their WT littermates were examined by immunohistochemistry. Scale bar: 50 μm.

    Techniques Used: Transfection, Plasmid Preparation, Construct, Activation Assay, Luciferase, Expressing, Immunofluorescence, Microscopy, Staining, Immunostaining, Immunohistochemistry



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    control gene fopflash  (Upstate Biotechnology Inc)
    90
    Upstate Biotechnology Inc control gene fopflash
    Catabolic regulation of LRP5 mediates via β-catenin-Tcf/Lef signaling. <t>(A)</t> <t>Chondrocytes</t> were transfected with 1 μg of empty vector (EV) or pSPORT6- Lrp5 plus the TOPflash or <t>FOPflash</t> reporter constructs. After 24 hours, transcriptional activation by β-catenin was determined by luciferase reporter gene assays ( n = 7 independent experiments). (B) Chondrocytes obtained from wild-type (WT) and Lrp5 -/- mice were treated with 1 ng/ml interleukin 1β (IL-1β), 50 ng/ml Wnt3a or 500 ng/ml Wnt7a, and transcriptional activation by β-catenin was evaluated by luciferase reporter gene assays ( n = 6). Values are expressed as means ± SEM (* P < 0.005). (C) β-catenin and LRP5 expression levels in cartilage after sham operation or destabilization of the medial meniscus (DMM) surgery were determined by immunofluorescence microscopy. 4′,6-diamidino-2-phenylindole (DAPI) staining was used for visualization of nuclei. Scale bar: 20 μm. (D) β-catenin, matrix metalloproteinase 3 (MMP3) and MMP13 expression levels in undamaged (Normal) and damaged (osteoarthritis; OA) human osteoarthritic cartilage were examined by immunostaining. (E) and (F) β-catenin, MMP3 and MMP13 expression levels in spontaneous (aging-induced) osteoarthritic cartilage and DMM-induced osteoarthritic cartilage from Lrp5 -/- mice and their WT littermates were examined by immunohistochemistry. Scale bar: 50 μm.
    Control Gene Fopflash, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control gene fopflash/product/Upstate Biotechnology Inc
    Average 90 stars, based on 1 article reviews
    control gene fopflash - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

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    Catabolic regulation of LRP5 mediates via β-catenin-Tcf/Lef signaling. (A) Chondrocytes were transfected with 1 μg of empty vector (EV) or pSPORT6- Lrp5 plus the TOPflash or FOPflash reporter constructs. After 24 hours, transcriptional activation by β-catenin was determined by luciferase reporter gene assays ( n = 7 independent experiments). (B) Chondrocytes obtained from wild-type (WT) and Lrp5 -/- mice were treated with 1 ng/ml interleukin 1β (IL-1β), 50 ng/ml Wnt3a or 500 ng/ml Wnt7a, and transcriptional activation by β-catenin was evaluated by luciferase reporter gene assays ( n = 6). Values are expressed as means ± SEM (* P < 0.005). (C) β-catenin and LRP5 expression levels in cartilage after sham operation or destabilization of the medial meniscus (DMM) surgery were determined by immunofluorescence microscopy. 4′,6-diamidino-2-phenylindole (DAPI) staining was used for visualization of nuclei. Scale bar: 20 μm. (D) β-catenin, matrix metalloproteinase 3 (MMP3) and MMP13 expression levels in undamaged (Normal) and damaged (osteoarthritis; OA) human osteoarthritic cartilage were examined by immunostaining. (E) and (F) β-catenin, MMP3 and MMP13 expression levels in spontaneous (aging-induced) osteoarthritic cartilage and DMM-induced osteoarthritic cartilage from Lrp5 -/- mice and their WT littermates were examined by immunohistochemistry. Scale bar: 50 μm.

    Journal: Arthritis Research & Therapy

    Article Title: Low-density lipoprotein receptor–related protein 5 governs Wnt-mediated osteoarthritic cartilage destruction

    doi: 10.1186/ar4466

    Figure Lengend Snippet: Catabolic regulation of LRP5 mediates via β-catenin-Tcf/Lef signaling. (A) Chondrocytes were transfected with 1 μg of empty vector (EV) or pSPORT6- Lrp5 plus the TOPflash or FOPflash reporter constructs. After 24 hours, transcriptional activation by β-catenin was determined by luciferase reporter gene assays ( n = 7 independent experiments). (B) Chondrocytes obtained from wild-type (WT) and Lrp5 -/- mice were treated with 1 ng/ml interleukin 1β (IL-1β), 50 ng/ml Wnt3a or 500 ng/ml Wnt7a, and transcriptional activation by β-catenin was evaluated by luciferase reporter gene assays ( n = 6). Values are expressed as means ± SEM (* P < 0.005). (C) β-catenin and LRP5 expression levels in cartilage after sham operation or destabilization of the medial meniscus (DMM) surgery were determined by immunofluorescence microscopy. 4′,6-diamidino-2-phenylindole (DAPI) staining was used for visualization of nuclei. Scale bar: 20 μm. (D) β-catenin, matrix metalloproteinase 3 (MMP3) and MMP13 expression levels in undamaged (Normal) and damaged (osteoarthritis; OA) human osteoarthritic cartilage were examined by immunostaining. (E) and (F) β-catenin, MMP3 and MMP13 expression levels in spontaneous (aging-induced) osteoarthritic cartilage and DMM-induced osteoarthritic cartilage from Lrp5 -/- mice and their WT littermates were examined by immunohistochemistry. Scale bar: 50 μm.

    Article Snippet: Chondrocytes were transfected with 1 μg of reporter gene (TOPflash) or control gene (FOPflash) (both from Upstate Biotechnology, Lake Placid, NY, USA) and 1 μg of pCMV - β-galactosidase using Lipofectamine 2000.

    Techniques: Transfection, Plasmid Preparation, Construct, Activation Assay, Luciferase, Expressing, Immunofluorescence, Microscopy, Staining, Immunostaining, Immunohistochemistry